Toxicology evaluation is a critical step in drug development and biomedical research. It helps determine the safety profile of new compounds by identifying potential harmful effects on biological systems. This involves both in vitro and in vivo testing to assess acute, subacute, and chronic toxicity. Parameters like LD50, organ histopathology, hematological changes, and biochemical markers are studied. Reliable toxicological data are essential before moving any compound into clinical trials, ensuring patient safety and regulatory compliance.